Composite
sam8sam5

Part:BBa_I742143:Design

Designed by: Chris French   Group: iGEM07_Edinburgh   (2007-10-23)


sam8+sam5 (tyrosine ammonia lyase + coumarate hydroxylase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2994
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2994
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 808
    Illegal BamHI site found at 1210
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2994
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2994
    Illegal NgoMIV site found at 551
    Illegal NgoMIV site found at 1595
    Illegal NgoMIV site found at 1603
    Illegal NgoMIV site found at 2353
    Illegal NgoMIV site found at 2526
    Illegal NgoMIV site found at 2928
    Illegal NgoMIV site found at 2932
    Illegal AgeI site found at 498
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 102
    Illegal BsaI site found at 368
    Illegal BsaI.rc site found at 2127


Design Notes

Note that this version of sam5 contains a PstI site near the 3' end. Thus this biobrick was prepared by inserting sam8 (EcoRI-SpeI) upstream of sam5 (EcoRI-XbaI) to avoid cutting with PstI.


Source

Both genes are derived from Saccharothrix espanaensis DSM 4429.

References